OT-2 Robot

SKU: 999-00111, 999-00002, 999-00002
4 weeks
999-00111, 999-00002, 999-00002
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About the product

OT-2—your personal pipetting robot

US and international lead time is 1 week.


The Opentrons® OT-2 is a high-precision, open-source liquid handler designed to be easy for scientists to use and flexible enough to automate many application workflows. This robot and its accompanying automation platform are used to automate hundreds of protocols and workflows in genomics, proteomics, cell-based assays, and drug discovery.  

The OT-2 has a user-friendly, plug and play graphical interface to design protocols compatible with any ANSI/SBS compliant or automation friendly labware. The base model includes an Opentrons GEN2 Single-Channel Pipette; you can also use up to two pipettes at the same time. Additional pipettes can be purchased separately and are easily interchangeable by the user. Consumables and other labware are sold separately.


  • Accuracy:  15% at 1uL
  • Precision:  5% at 1uL
  • Throughput: Fast fill a 96-well plate in 22 seconds  

Performance Tables 

(found here:  OT-2 Pipettes by Opentrons | New Pipettes for Lab Automation | Opentrons)


Key Features

  • Flexible open-source platform 
    • Self-serve unboxing and installation 
    • Reagent agnostic
    • Plug-and-play setup with access to protocol libraries to enable immediate assay implementation
    • Easily integrates to your existing instruments
    • Compatible with ANSI/SBS compliant or automation friendly tubes, microplates, deep-well plates, reservoirs and adapters
  • Supports Ethernet or USB connection (external dongle provided)
  • Easy access to electronics via external PCBA enclosure
  • 4 USB ports available to support connection to multiple modules
  • LED indicator provide users feedback, diagnosis & troubleshooting aid on instrument power state, system processing, ethernet and network connectivity

Ideal Use

  • Genomics 
  • Proteomics
  • Cell-based Assays
  • Biochemical Assays
  • Drug Discovery

Dimensions:  63cm x 57cm x 66cm / 25in x 22.5in x 26in (W,D,H)


Product Compliance

  • Tested to UL 61010-1 safety standard 
  • Complies to the following EU Directives in order to be marked with the CE mark
    • Low Voltage Directive
    • Machinery Directive
    • ROHS Directive
    • EMC Directive
  • CB certificate 


Pipette Configurations
Single and multi-channel pipetting. 2-pipette mounts, for a configuration of 1 or 2 single or 8-channel pipettes. Pipettes are easily interchangeable.

Pipette volumes and specs 
Single channel: 1-1000 µl
8-channel: 1-300 µl
OT-2 GEN2 Pipettes

# of deck slots
11 deck slots enable countless configurations. Deck slots are compatible with standard SBS dimensions. Deck also includes a removable trash bin.  

WiFi 2.4 GHz IEEE 802.11b/g/n, USB 2.0. Learn more.

40kg or 88lb

Video & Audio
Integrated video camera and audio system included for future or custom functionality

Power Requirements
100-240 VAC / 50-60 Hz
220 W MAX

Frame Composition
Rigid steel and CNC aluminum design

Operating Temperature & Relative Humidity
Recommended 20-24C. Recommended 40-60% RH. Learn more.

Fills a 96-well plate in 22 seconds. View Plate Fill Run Time.

Full polycarbonate enclosure designed to limit exposure. The OT-2 is not a sterile environment. Add your own UV lights, or the Opentrons HEPA Module

Plate Type Compatibility
Use 96 or 384 well plates. Use 96-well plates for optimal deck compatibility & touch tips. Compatible with up to 384-well plates.

API Compatibility
Interfaces with any product that has an API. 
Opentrons software is open-source and users can make adjustments to the software as needed to interface with other products. While all integrations are possible, they all require custom code work i.e. most customers will need to write custom libraries and install software packages that aren't shipped with the robot. Learn more.

Windows 10, macOS 10.10 or later, Ubuntu 12.04 or later. View the Opentrons App

Tip Compatibility
Use Opentrons Tips and Opentrons Filter Tips. Opentrons guarantees the performance of Opentrons Tips which were designed specifically for the OT-2. For customers willing to accept potentially lower performance, 3rd party universal tips can be used as well. 

Labware Compatibility
Use common labware already in our Labware Library, or add your own. If your labware fits on the OT-2 but is not in the Labware Library, it can be added by you using our Labware Creator, or by our team. Learn more.



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